英文名称：Protease S Aureus；Endoproteinase Glu-C from Staphylococcus aureus V8；V8 Protease

中文名称：蛋白酶S

CAS号：66676-43-5

级别：BR

来源：Staph aureus V8

Activity：≥500units/mg

蛋白酶S活力定义：One Unit causes a change of 0.001 A280 nm per minute at 37℃, pH 7.8 using casein as the substrate

产品描述：Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons ａnd optimum pH＇s of 4.0 ａnd 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate ａnd monovalent anions such as F-, Cl-, CH3COO- ａnd NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).

性状：粉末。

用途：生化研究。

保存：2～8℃